Methemoglobin reductase activity of nuclear erythrocytes of the sea ruff (<i>Scorpaena porcus</i>, Linnaeus, 1758) under normal and oxidative stress conditions (<i>in vitro</i> experiments)

Soldatov, A. A.

doi:10.31857/S1026347025010022

PII

S1026347025010022-1

DOI

10.31857/S1026347025010022

Publication type

Article

Status

Published

Authors

A. A. Soldatov

Affiliation: Federal Research Center “A.O. Kovalevsky Institute of Biology of the South Seas of the Russian Academy of Sciences”

Volume/ Edition

Volume / Issue number 1

Pages

16-23

Abstract

A method for evaluating the methemoglobin reductase activity of nuclear erythrocytes of teleost fish is proposed. The work was performed on a bottom marine species (Scorpaena porcus, Linnaeus, 1758). In an in vitro experiment, the erythrocyte suspension of this type was weighed in solutions with different concentrations of NaNO₂, after washing from this compound, the process of methemoglobin (MetHb) reduction was studied for 150 min. The functional state of hemoglobin was judged by the results of spectral analysis. The study of the kinetics of MetHb reduction showed that the dependence was well described by the equation of an exponential function with a coefficient of determination (R²) greater than 0.9. The nature of the dependence remained at different levels of oxidative stress. This made it possible to calculate the velocity constant of the first order k (25 ^oC). In this species, it was 5.75–6.45 (10^–3) min^–1, which slightly exceeded the known values for mammals, and was close to the data obtained for other species of bony fish. It was found that the MetHb-reductase activity of the nuclear erythrocytes of the sea ruff increased with a rise in the oxidative load. At a concentration of 6.0 mM NaNO₂, it was 1.73 ± 0.21 µM MetHb min^–1 g^–1 Hb. It is assumed that this is due to the process of deoxygenation of hemoglobin.

Keywords

метгемоглобин окислительная нагрузка MetHb-редуктазная активность ядерные эритроциты Scorpaena porcus

Date of publication

04.11.2025

Year of publication

2025

Number of purchasers

Views

References

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GOST	Soldatov A. Methemoglobin reductase activity of nuclear erythrocytes of the sea ruff (Scorpaena porcus, Linnaeus, 1758) under normal and oxidative stress conditions (in vitro experiments) // Biology Bulletin. – 2025. – Issue number 1 C. 16-23 . URL: https://serbiolras.ru/s1026347025010022-1/?version_id=107254. DOI: 10.31857/S1026347025010022
MLA	Soldatov, A. A "Methemoglobin reductase activity of nuclear erythrocytes of the sea ruff (Scorpaena porcus, Linnaeus, 1758) under normal and oxidative stress conditions (in vitro experiments)." Biology Bulletin. 1 (2025).:16-23. DOI: 10.31857/S1026347025010022
APA	Soldatov A. (2025). Methemoglobin reductase activity of nuclear erythrocytes of the sea ruff (Scorpaena porcus, Linnaeus, 1758) under normal and oxidative stress conditions (in vitro experiments). Biology Bulletin. no. 1, pp.16-23 DOI: 10.31857/S1026347025010022

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Methemoglobin reductase activity of nuclear erythrocytes of the sea ruff (Scorpaena porcus, Linnaeus, 1758) under normal and oxidative stress conditions (in vitro experiments)

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RAS BiologyИзвестия Российской академии наук. Серия биологическая Biology Bulletin

Methemoglobin reductase activity of nuclear erythrocytes of the sea ruff (Scorpaena porcus, Linnaeus, 1758) under normal and oxidative stress conditions (in vitro experiments)

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